SOX-4; RBX-100UG

Code: AB5803 D2-231

Application

Anti-Sox4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Sox4 & has been validated in WB, ICC.

Immunogen

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Your Price
€429.00 EACH
€527.67 inc. VAT

Application

Anti-Sox4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Sox4 & has been validated in WB, ICC.

Immunogen

Synthetic peptide, amino acids 34-50 of Sox4.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB9092

Specificity

Recognizes Sox4. The molecular weight of the protein is 47,258 Daltons.

Storage and Stability

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (~10,000G) for 15 minutes to pellet the precipitated antibody product. There will be a whitish pellet near the bottom. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 µL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 µg of precipitated antibody, the amount of buffer needed would be 50 µL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

antibody formaffinity purified immunoglobulin
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... SOX4(6659)
manufacturer/tradenameChemicon®
NCBI accession no.NM_003107.2
purified byaffinity chromatography
Quality Level100
shipped indry ice
species reactivitymouse, human
technique(s)immunocytochemistry: suitable, western blot: suitable
UniProt accession no.Q06945
This product has met the following criteria to qualify for the following awards:



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